CRISPR (clustered regularly interspaced short palindromic repeats) utility relies on a stable Cas effector complex binding to its target site. However, a Cas complex bound to DNA may be removed by motor proteins carrying out host processes and the mechanism governing this removal remains unclear. Intriguingly, during CRISPR interference, RNA polymerase (RNAP) progression is only fully blocked by a bound endonuclease-deficient Cas (dCas) from the protospacer adjacent motif (PAM)-proximal side.

Cellular response to stimulation governs tissue scale processes ranging from growth and development to maintaining tissue health and initiating disease. To determine how cells coordinate their response to such stimuli, it is necessary to simultaneously track and measure the spatiotemporal distribution of their behaviors throughout the tissue. Here, we report on a novel SpatioTemporal Response Analysis IN Situ (STRAINS) tool that uses fluorescent micrographs, cell tracking, and machine learning to measure such behavioral distributions.

Many biological tissues feature a heterogeneous network of fibers whose tensile and bending rigidity contribute substantially to these tissues' elastic properties. Rigidity percolation has emerged as an important paradigm for relating these filamentous tissues' mechanics to the concentrations of their constituents. Past studies have generally considered tuning of networks by spatially homogeneous variation in concentration, while ignoring structural correlation.

Moiré superlattice systems such as transition metal dichalcogenide heterobilayers have garnered significant recent interest due to their promising utility as tunable solid state simulators. Recent experiments on a WSe2/WS2 heterobilayer detected incompressible charge ordered states that one can view as generalized Wigner crystals. The tunability of the transition metal dichalcogenide heterobilayer Moiré system presents an opportunity to study the rich set of possible phases upon melting these charge-ordered states.

In its ground state, RuO2 was long thought to be an ordinary metallic paramagnet. Recent neutron and x-ray diffraction revealed that bulk RuO2 is an antiferromagnet with TN above 300 K. Furthermore, epitaxial strain induces superconductivity in thin films of RuO2 below 2 K. Here, we present a resonant elastic x-ray scattering study at the RuL2 edge of the strained RuO2 films exhibiting the strain-induced superconductivity. We observe an azimuthal modulation of the 100 Bragg peak consistent with bulk.

We present in situ calorimetry, thermal conductivity, and thermal diffusivity measurements of materials using temperature-sensing optical wireless integrated circuits (OWiCs). These microscopic and untethered optical sensors eliminate input wires and reduce parasitic effects. Each OWiC has a mass of ∼100 ng, a 100-μm-scale footprint, and a thermal response time of microseconds. We demonstrate that they can measure the thermal properties of nearly any material, from aerogels to metals, on samples as small as 100 ng and over thermal diffusivities covering four orders of magnitude.

The onset of rigidity in interacting liquids, as they undergo a transition to a disordered solid, is associated with a rearrangement of the low-frequency vibrational spectrum. In this Letter, we derive scaling forms for the singular dynamical response of disordered viscoelastic networks near both jamming and rigidity percolation. Using effective-medium theory, we extract critical exponents, invariant scaling combinations, and analytical formulas for universal scaling functions near these transitions.

Nanoscale spin textures, especially magnetic skyrmions, have attracted intense interest as candidate high-density and power-efficient information carriers for spintronic devices1,2. Facilitating a deeper understanding of sub-hundred-nanometre to atomic-scale spin textures requires more advanced magnetic imaging techniques3–5. Here we demonstrate a Lorentz electron ptychography method that can enable high-resolution, high-sensitivity magnetic field imaging for widely available electron microscopes.

We develop a theory for a continuous bandwidth-tuned transition at fixed fractional electron filling from a metal with a generic Fermi surface to a "Wigner-Mott"insulator that spontaneously breaks crystalline space-group symmetries. Across the quantum critical point, (i) the entire electronic Fermi surface disappears abruptly upon approaching from the metallic side, and (ii) the insulating charge gap and various order parameters associated with the spontaneously broken space-group symmetries vanish continuously upon approaching from the insulating side.

Models for nonunitary quantum dynamics, such as quantum circuits that include projective measurements, have recently been shown to exhibit rich quantum critical behavior. There are many complementary perspectives on this behavior. For example, there is a known correspondence between d-dimensional local nonunitary quantum circuits and tensor networks on a [D=(d+1)]-dimensional lattice.