Unzipping single DNA molecules to study nucleosome structure and dynamics
Abstract
DNA unzipping is a powerful tool to study protein-DNA interactions at the single-molecule level. In this chapter, we provide a detailed and practical guide to performing this technique with an optical trap, using nucleosome studies as an example. We detail protocols for preparing an unzipping template, constructing and calibrating the instrument, and acquiring, processing, and analyzing unzipping data. We also summarize major results from utilization of this technique for the studies of nucleosome structure, dynamics, positioning, and remodeling. © 2012 Elsevier Inc.
Date Published
Journal
Methods in Enzymology
Volume
513
Number of Pages
29-58,
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865472310&doi=10.1016%2fB978-0-12-391938-0.00002-1&partnerID=40&md5=41c29dbc5c8c84daec2259e0cc6a963d
DOI
10.1016/B978-0-12-391938-0.00002-1
Research Area
Group (Lab)
Michelle Wang Group