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Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source

Cornell Affiliated Author(s)

Author

A. Munke
J. Andreasson
A. Aquila
S. Awel
K. Ayyer
A. Barty
R.J. Bean
P. Berntsen
J. Bielecki
S. Boutet
M. Bucher
H.N. Chapman
B.J. Daurer
H. Demirci
V. Elser
P. Fromme
J. Hajdu
M.F. Hantke
A. Higashiura
B.G. Hogue
A. Hosseinizadeh
Y. Kim
R.A. Kirian
H.K.N. Reddy
T.-Y. Lan
D.S.D. Larsson
H. Liu
N.D. Loh
F.R.N.C. Maia
A.P. Mancuso
K. Mühlig
A. Nakagawa
D. Nam
G. Nelson
C. Nettelblad
K. Okamoto
A. Ourmazd
M. Rose
G. Van Der Schot
P. Schwander
M.M. Seibert
J.A. Sellberg
R.G. Sierra
C. Song
M. Svenda
N. Timneanu
I.A. Vartanyants
D. Westphal
M.O. Wiedorn
G.J. Williams
P.L. Xavier
C.H. Yoon
J. Zook

Abstract

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

Date Published

Journal

Scientific Data

Volume

3

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84982746851&doi=10.1038%2fsdata.2016.64&partnerID=40&md5=fb179f493e4610da13be044d30d5918a

DOI

10.1038/sdata.2016.64

Group (Lab)

Veit Elser Group

Funding Source

R01GM097463

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