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Cryogenic x-ray diffraction microscopy utilizing high-pressure cryopreservation

Cornell Affiliated Author(s)

Author

E. Lima
Y. Chushkin
P. Van Der Linden
C.U. Kim
F. Zontone
P. Carpentier
Sol Gruner
P. Pernot

Abstract

We present cryo x-ray diffraction microscopy of high-pressure-cryofixed bacteria and report high-convergence imaging with multiple image reconstructions. Hydrated D. radiodurans cells were cryofixed at 200 MPa pressure into ∼10-μm-thick water layers and their unstained, hydrated cellular environments were imaged by phasing diffraction patterns, reaching sub-30-nm resolutions with hard x-rays. Comparisons were made with conventional ambient-pressure-cryofixed samples, with respect to both coherent small-angle x-ray scattering and the image reconstruction. The results show a correlation between the level of background ice signal and phasing convergence, suggesting that phasing difficulties with frozen-hydrated specimens may be caused by high-background ice scattering. © 2014 American Physical Society.

Date Published

Journal

Physical Review E - Statistical, Nonlinear, and Soft Matter Physics

Volume

90

Issue

4

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908424087&doi=10.1103%2fPhysRevE.90.042713&partnerID=40&md5=d23135aecb78fc282485ba3fe7567a36

DOI

10.1103/PhysRevE.90.042713

Group (Lab)

Sol M. Gruner Group

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